My primary function is to serve as Administrator/Manager of the Flow Cytometry-Confocal Microscopy Core Facility, a research support resource which is overseen by the School of Medicine Office of Research and Graduate Studies. Flow cytometry and confocal microscopy are powerful techniques based on laser light excitation of fluorescent molecules. Flow cytometry is commonly used to study various aspects of cell function, including apoptosis, cell cycle, and immunophenotype analysis. The facility has bench top flow analyzers (11-color Becton Dickinson LSR II and three-color FACScan), and a five-color cell sorter (FACS Vantage) to isolate specific cell subsets from a complex mixture of cell types. Confocal microscopy (Zeiss LSM 510 Laser-scanning Confocal Microscope) is used primarily for cellular co-localization studies and three-dimensional reconstruction.
My research interest is focused on development of flow cytometric and confocal microscopic methods to study the role of cell membrane biomolecule interactions (proteins, glycans, and lipids) in health and disease. I have several collaborative research projects in progress with other ECU faculty (see recent publications below).